WecStrain™ Probiotic Agents Lacticaseibacillus casei LC16 and Bifidobacterium longum subsp. infantis BI03 Targeting Reproductive Tract Aging

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Background:

As individuals age, the reproductive tract undergoes natural degenerative changes, leading to an altered microenvironment and imbalances in microbial communities. These changes can decrease reproductive functionality and increase the risk of associated diseases. Conventional treatments often rely on hormone replacement therapy, which carries risks of side effects and does not fundamentally restore the natural balance of the reproductive system’s microenvironment. The proposed solution in this patent involves using probiotics, which are known to regulate the body's microecological balance safely and effectively.

Probiotic Agent Composition:

The described probiotic agent consists of:

· Lacticaseibacillus casei LC16 (CGMCC No.24110)

· Bifidobacterium longum subsp. infantis BI03 (CGMCC No.24473)

The active bacterial count in the formulation is at least 1×10¹⁰ CFU per mL or gram. The formulation is designed to be used in various product types, including freeze-dried powders, capsules, tablets, or granules.

Method of Preparation:

1. Fermentation Process: The LC16 and BI03 strains are separately cultured in a medium. After fermentation, the resulting liquid is centrifuged and mixed with cryoprotectants to undergo freeze-drying. The probiotic powders are then combined in specified proportions.

2. Cryoprotectants: These include substances such as skimmed milk, gelatin, dextrin, or arabic gum to ensure stability during freeze-drying.

3. Functional Additives: The formulation can also include prebiotics like fructooligosaccharides or galactooligosaccharides to enhance the probiotics' efficacy.

Key Benefits and Mechanisms:

The probiotic formulation targets reproductive tract aging through:

· pH Balance: The probiotics lower vaginal pH, restoring the acidic environment conducive to health.

· Oxidative Stress Reduction: The agents reduce oxidative stress markers, including malondialdehyde (MDA), and regulate reactive oxygen species (ROS), both of which are elevated in aging tissues.

· Hormonal Regulation: The probiotics help normalize reproductive hormones like estrogen and luteinizing hormone (LH), whose imbalances are associated with aging and reproductive dysfunction.

· Immunomodulation: The formulation boosts immune responses, as indicated by an increase in immunoglobulin A (IgA) in vaginal fluids.

Applications:

The probiotic formulation has several therapeutic applications:

· Female Reproductive Health: It can be used to create products that promote general reproductive health in women.

· Prevention and Relief of Aging: The agent can be applied to products aimed at preventing or alleviating signs of aging in the reproductive tract.

· Therapeutic Use for Damage: The formulation can also be utilized in medicines to prevent, mitigate, or treat reproductive tract damage.

Conclusion:

This patented probiotic agent offers a promising new strategy for addressing reproductive tract aging through the synergistic action of Lacticaseibacillus casei LC16 and Bifidobacterium longum subsp. infantis BI03. It is safe, effective, and can be applied in various health products to promote reproductive health, prevent aging, and treat related conditions.

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